Nanoscale optomechanical actuators for controlling mechanotransduction in living cells
Z. Liu, Y. Liu, Y. Chang, H. R. Seyf, A. Henry, A. L. Mattheyses, K. Yehl, Y. Zhang, Z. Huang and K. Salaita, Nanoscale optomechanical actuators for controlling mechanotransduction in living cells, Nature Methods, doi:10.1038/nmeth.3689 (2015).
To control receptor tension optically at the cell surface, we developed an approach involving optomechanical actuator nanoparticles that are controlled with near-infrared light. Illumination leads to particle collapse, delivering piconewton forces to specific cell surface receptors with high spatial and temporal resolution. We demonstrate optomechanical actuation by controlling integrin-based focal adhesion formation, cell protrusion and migration, and T cell receptor activation.